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Confocal and Advanced Light Microscopy Facility

CMVM, QMRI, UNIVERSITY OF EDINBURGH

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Facility

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The CALM facility

The CALM facility provides two confocal laser scanning microscopes, which are located in room C2.39 on the first floor of the Queen's Medical Research Institue (QMRI).

They are complemented by two widefield fluorescence microscopes, for live cell imaging and for standrad image acquisition from fixed samples.

Users have to book the microscopes beforehand using the on-line booking system and appropriate charges apply.

 

Leica SP5 C - laser scanning confocal microscope

This system is built on a DMI6000CS inverted microscope base with a conventional SP5 II scanhead. It provides 8 different laser lines for excitation (405, 458, 476, 488, 514, 543, 596, 633 nm). Its unique design allows filter-free separation of different spectral channels as well as spectral unmixing (3 detectors). This system provides all standard functions for multi-dimensional imaging and is the microscope of choice for high resolution images from fixed samples. More details

Zeiss LSM 510 META - laser scanning confocal microscope

This is a microscope system based on an inverted, motorised Axiovert 200M stand with a LSM510 META scanhead.  It provides 6 laser lines for fluorophore excitation (458, 477, 488, 514, 543, 633 nm) and allows multi-dimensional image acquisition. The microscope is eqipped with incubation chambers that encloses the stage. This allows the control of the temperature, air humidity and carbon dioxide concentration on the stage, a prerequisite for live cell imaging and the microscopic studies of physiological processes. More details

Leica DMi8 widefield fluorescence microscope for live cell imaging

This microscope is ideal for image acquisition from thin live samples such as cell monolayers. The filtering allows fast imaging of the standard fluorescent proteins such as CFP, YFP, mCherry and GFP using a sCMOS camera. Physical autofocus facilitates live cell imaging with a range of water-corrected objectives. More details

Andor Revolution XDi spinning disk confocal microscope for fast image acquisition and photo-manipulation

This imaging platform is based on an inverted Olympus IX83 with a Yokogawa CSU-X1 module. The system includes a fast emission filter wheel, an adiditonal beam splitter and two highly sensitive EMCCD cameras. A MicroPoint laser module allows independent photo-manipulation of the sample. The entire system is enclosed and environmentally controlled. More details

LaVision Biotec TriM scope II intra-vital imaging platform

This system is a custom-built platform for multi-photon excitation-based deep penetration imaging. It is ideal for imaging live specimens deeper into tissues. It is built around an inverted Nikon TiU eclipse inverted stand and a LaVision intravital upright stage to accommodate a wide range of samples and specimens. High NA lenses with long working distances guarantee good optical penetration of the sample. The system is also equipped with a resonant scanner for faster imaging. It also provides modules for FLIM, photoactivation and wave front modulation. For more information please contact Rolly Wiegand.

 

Last update:25 June 2017

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